Fig. 4

miR-508-3p is down-regulated in GC cell lines and has tumor suppressor potential. a The expression of miR-508-3p in ten GC cell lines compared with commercial normal gastric total RNA (AM7996, Ambion). b 6-day MTT proliferation assays revealed the growth-inhibition effect of miR-508-3p in MKN28, MGC-803 and SGC-7901 cells (**, P < 0.001). c Ectopic miR-508-3p expression decreased monolayer colony formation in all the three GC cell lines (**, P < 0.001). The experiments were performed in three wells to get SDs. d The GC cell invasion ability was significantly inhibited by ectopic expression of miR-508-3p (*, P < 0.05). The invaded cells from the matrigel were counted in three random vision fields for getting SDs. e The mRNA expression of CCND1 and MMP9 was down-regulated upon ectopic miR-508-3p expression in MKN28, MGC-803, and SGC-7901 cells (**, P <0.001)