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Fig 3 | Molecular Cancer

Fig 3

From: Key role of MEK/ERK pathway in sustaining tumorigenicity and in vitro radioresistance of embryonal rhabdomyosarcoma stem-like cell population

Fig 3

Effects of inhibition of p38 on sphere formation, stem and differentiative markers expression. RD cells were incubated in SC medium for 7 days (CTR) in the absence or in the presence of 2.5 μM SB203580 (SB) without or with 10 μM U0126 (SB + U). a Microphotographs (Bar = 100 μm) and cell cycle analysis of a representative experiment (out of three). b Histogram with cell numbers determined by cell counting. c Histograms of percentage of CD133 and CXCR4 positive cells determined by FACS analysis. Values represent the mean ± SD of 3 independent experiments. Statistical significance is indicated (**p < 0.01). d Western blot analysis of protein lysates. The expression levels of Nanog, ERK1/2-PO4, ERK1/2, myogenin, p38-PO4 and p38 were analyzed. Protein bands were quantified by densitometry with respect to GAPDH, ERK1/2

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