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Fig. 3 | Molecular Cancer

Fig. 3

From: ERK mediated upregulation of death receptor 5 overcomes the lack of p53 functionality in the diaminothiazole DAT1 induced apoptosis in colon cancer models: efficiency of DAT1 in Ras-Raf mutated cells

Fig. 3

ERK activation and its nuclear translocation by DAT1 a. Cells were treated with DAT1 for the indicated time periods and immunoblotted with the respective antibodies. Band intensities of P-p38, P-ERK and P-SAPK/JNK were quantified and were normalized with the levels of p38, ERK and SAPK/JNK respectively. b. Nuclear translocation of ERK was checked in HCT116 cells by immunofluoresence. Cells were treated with DAT1 for 24 h, fixed and probed with P-ERK antibody followed by alexaflour 488. Nuclei were stained with propidium iodide and imaged using a confocal microscope. c. Nuclear extract was collected from HCT116 cells and immunoblotting was performed using P-ERK antibody. Lamin was used as the nuclear loading control

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Molecular Cancer

ISSN: 1476-4598