Fig. 2

Bit1 shRNA efficiently reduced Bit1 protein level in EC9706 and TE1 cells. Total protein was extracted from EC9706 and TE1 cells untreated and transfected with Bit1 shRNA and negative shRNA at different time points, and Western blotting was used to investigate the Bit1 protein level. a Western blotting assay for Bit1 protein level in untreated group, negative group and Bit1 shRNA group at different time points including days 1, 2, 3, 4, 5, 6 and 7 in EC9706 cells. b The relative Bit1 protein level was counted by the ratio of Bit1 level to β-actin level in EC9706 cells, and the results from the three independently repeated experiments, expressed as means ± SD, were investigated by SPSS 17.0 statistical software, *P < 0.05, compared with untreated EC9706 cells, negative group, EC9706 cells on days 6 and 7 after transfection with Bit1 shRNA, **P < 0.05, compared with the other groups, # P < 0.05, compared with EC9706 cells on day 5. c Western blotting assay for Bit1 protein level in untreated group, negative group and Bit1 shRNA group at different time points including days 1, 2, 3 and 4 in TE1 cells. d The relative Bit1 protein level was counted by the ratio of Bit1 level to β-actin level in TE1 cells, and the results from the three independently repeated experiments, expressed as means ± SD, were investigated by SPSS 17.0 statistical software, *P < 0.05, compared with untreated EC9706 cells and negative group, **P < 0.05, compared with the other groups