Fig. 4From: Bit1 knockdown contributes to growth suppression as well as the decreases of migration and invasion abilities in esophageal squamous cell carcinoma via suppressing FAK-paxillin pathwayKnockdown of Bit1 expression promotes EC9706 and TE1 cell apoptosis. After transfected with pSilencer3.1-H1-neo-Bit1-shRNA or pSilencer3.1-H1-neo-negative-shRNA for 72 h, cells were subjected to Cell Death ELISA. Three independent experiments were performed. a ELISA assay for cell apoptosis in EC9706 cells; b ELISA assay for cell apoptosis in TE1 cells; c Flow cytometry assay for cell apoptosis in EC9706 and TE1 cells; d Three independently repeated experiment for cell apoptosis in EC9706 cells; e Three independently repeated experiment for cell apoptosis in TE1 cells; *P < 0.05 as compared to untreated and negative groupsBack to article page