Fig. 5
Bit1 shRNA significantly suppressed tumor growth in EC9706 xenografted nude mice. Bit1 shRNA mediated the inhibitory effect on tumor growth in EC9706 xenografted nude mice. EC9706 cells (4 × 106 per nude mouse) were inoculated into the back of right flank of each nude mouse. When tumor volume reached approximately 100 mm3 (about on day 5), nude mice were randomly split into four groups: 1: negative (5 μg), 2: negative (10 μg), 3: shRNA (5 μg) and 4: shRNA (10 μg), and treatment and tumor volume measurement were performed every 4 days. The mice in different groups were injected via intratumor route with the different complexes, which consists of pSilencer3.1-H1-neo-Bit1-shRNA (5 μg and 10 μg) or pSilencer3.1-H1-neo-negative-shRNA (5 μg and 10 μg) and corresponding volume of Lipofectamine 2000. When measurement was terminated, the animals were sacrificed on day 21, and tumor tissues were collected. Finally, tumor growth curve was made to evaluate the effect of Bit1-shRNA on tumor growth. a Sacrificed animals and corresponding tumors in different groups. b Tumor growth curve in different groups. *P < 0.05, compared with negative groups and shRNA group (5 μg)