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Fig. 1 | Molecular Cancer

Fig. 1

From: GLI pathogenesis-related 1 functions as a tumor-suppressor in lung cancer

Fig. 1

GLIPR1 expression was suppressed by WDR77 in lung and prostate cancer cells. a DNA microarray and RT-RCR analysis of GLIPR1 mRNA expression in A549 cells. Fold-increase = GLIPR1 mRNA signals of cells expressing WDR77 shRNA/GLIPR1 signals of cells expressing NT shRNA. b Western Blot analysis of whole-cell lysates derived from A549 cells expressing NT shRNA (lane 1) or WDR77 shRNA (lane 2) with anti-WDR77, anti-GLIPR1 or anti-actin antibodies as indicated. c RT-RCR analysis of GLIPR1 mRNA expression in LNCaP, PC14, or PC3 cells. Fold-increase = GLIPR1 mRNA signals of cells expressing WDR77 shRNA/GLIPR1 signals of cells expressing NT shRNA. d Western Blot analysis of whole-cell lysates derived from PC14, LNCaP, or PC3 cells expressing NT shRNA (lane 1, 3, 5) or WDR77 shRNA (lane 2, 4, 6) with anti-WDR77, -CLIPR1, or -actin antibody as indicated

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