Fig. 5
From: GLI pathogenesis-related 1 functions as a tumor-suppressor in lung cancer
GLIPR1 inhibits cell proliferation. a Western blot analysis of whole-cell lysates derived from A549 cells infected with the control lentivirus (lane 1) or with variable amounts (50, 100, or 200 μl) of lentivirus expressing GLIPR1 (lanes 2–4). b Growth curves of control A549 cells or A549 cells expressing different levels of GLIPR1. c A549 cells infected with control lentivirus or GLIPR1-expressing lentivirus were allowed to grow in the presence of BrdU and immunostained with the anti-BrdU antibody (brown). The green arrow (left-hand panel) indicates the BrdU-negative staining cell and the black arrows (right-hand panel) indicate the BrdU-positive staining cells. d Percentage of BrdU-positive cells in A549 cells infected with control lentivirus or GLIPR1-expressing lentivirus. The results represent the means of three independent experiments ± the standard error of the mean (SEM). *, P<0.05; **, P<0.01; ***, P<0.001