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Fig. 6 | Molecular Cancer

Fig. 6

From: ITGA6 is directly regulated by hypoxia-inducible factors and enriches for cancer stem cell activity and invasion in metastatic breast cancer models

Fig. 6

Enriching for CD49f by cell sorting enhances cell invasion in MDA-MB-231 cells. a Mock-sorted, CD49fHigh or CD49fLow MDA-MB-231 cell populations were exposed for 24–48 h to hypoxia and invasion then compared (n = 3-4 replicate wells/population/experiment; data are representative of 3 independent sort experiments); p-values calculated by Student’s t-test are shown. The grand mean invasion index ± SEM is expressed relative to Mock-sorted cells (cells stained with CD49f-FITC but not gated during sort; set to 1.0). b IF staining for CD49f-FITC and HIF-1α in cytospun CD49fHigh, CD49fLow or Mock sorted cells counterstained with DAPI; scale bar = 50 μm. c qRT-PCR analysis to compare expression levels of known HIF-dependent target genes in the CD49fHigh vs. CD49fLow cell populations; all values were first normalized for loading and were then normalized to Mock sorted cells (white bars; fold change, FC set to 1.0). The mean ± SEM is reported for three independent experiments

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