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Fig. 1 | Molecular Cancer

Fig. 1

From: Reciprocal activation between STAT3 and miR-181b regulates the proliferation of esophageal cancer stem-like cells via the CYLD pathway

Fig. 1

Identification of esophageal cancer stem cell-like cell sphere formation with stemness-related features in serum-free DMEM/F12 medium (SFDM). a Eca109 and Eca9706 cells were cultured in SFDM, leading to tumor sphere formation. Tumor sphere formation was propagated by enzymatic dissociation. Cells cultured in regular medium did not form tumor spheres. b Flow cytometry analysis of CD44 and CD24 expressed in Eca109 and Eca9706 parental cells and their tumor spheres. c, d Stemness factor expression conditions in Eca109 and Eca9706 parental cells and their tumor spheres quantified by qPCR analysis. e, f qPCR analysis of mesenchymal trait markers expressed in Eca109 and Eca9706 parental cells and their tumor spheres. Error bars represent mean ± SD

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