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Fig. 2 | Molecular Cancer

Fig. 2

From: Reciprocal activation between STAT3 and miR-181b regulates the proliferation of esophageal cancer stem-like cells via the CYLD pathway

Fig. 2

Analysis of proliferation capacity and tumorigenicity in vivo. a Colony formation assay detection of Eca109 parental cells and tumor sphere cells. Colonies were counted following 0.5 % crystal violet staining. Triplicate independent experiments were performed. Scale bar = 10 mm. b Animal experiments for detection of tumorigenicity of Eca109 cells and tumor sphere cells. Subcutaneous tumors formed by Eca109 parental cells and tumor sphere cells. c qPCR analysis of CD44 and CD24 in mouse tumor formation derived from Eca109 parental cells and SFCs. d mRNA expression of ABCB1, ABCG2, and MRP1 in mice tumors derived from Eca109 parental cells and SFCs. SFCs, sphere formation cells. Error bars represent mean ± SD

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