Fig. 1
Sorafenib increases the expression levels of multiple MT1 genes in cancer cells. a MT1 genes represent a significant fraction (6/35) of the genes found to be overexpressed by more than three-fold in Huh7 cells exposed to sorafenib (10 μM) for 9 h. b QPCR analysis of the mRNA encoding the main isoforms of MT1 in Huh7 cells exposed to sorafenib (10 μM) for 9 h. c Immunoblot analysis of the expression of the MT1 protein. Protein extracts were prepared from Huh7 cells treated for 4 to 18 h with 10 μM sorafenib. d MT1G mRNA levels were measured by QPCR in a panel of cancer cell lines and in primary human hepatocytes (PHH) exposed to sorafenib 10 μM for 9 h. *: p < 0.05 compared to control, n.s.: non-significant compared to control. e: MT1G mRNA levels analysis in Huh7 cells exposed to ZnCl2 100 μM and sorafenib (10 μM) for 18 h. f Analysis of MT1G mRNA levels by QPCR in Huh7 cell lines exposed to sorafenib or selected kinase inhibitors applied at pharmacological concentrations (selumetinib 1 μM, erlotinib 1 μM, vemurafenib 10 μM and rapamycin 1 μM) for 18 h. *: p < 0.05 compared to control