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Fig. 4 | Molecular Cancer

Fig. 4

From: Metallothionein-1 as a biomarker of altered redox metabolism in hepatocellular carcinoma cells exposed to sorafenib

Fig. 4

Role of MT1G in Huh7 cells. a Induction of MT1G mRNA in Huh7 cells transfected with MT1G siRNA and control siRNA, exposed to sorafenib (10 μM, 18 h). *: p < 0.05 compared to control siRNA treated with sorafenib. b Analysis of the effects of sorafenib (10 μM, 18 h) on its main target kinases (as reflected by the activation levels of the RAF-MEK-ERK cascade) in Huh7 cells transfected with control siRNA or siRNA directed against MT1G. c: Ferroptosis induction by sorafenib. A value of % released LDH was calculated for each condition in Huh7 transfected with control siRNA vs MT1G siRNA, and cells were treated with sorafenib (10 μM, 18 h) as indicated. ns: non-significant compared to control siRNA exposed to sorafenib. d Clonogenic growth of Huh7 cells exposed to sorafenib applied at different concentrations (0.5, 1, 2, 5 and 10 μm) after transfection with the indicated siRNA

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Molecular Cancer

ISSN: 1476-4598