Fig. 5
From: Rad51C-ATXN7 fusion gene expression in colorectal tumors
Western blot analysis of Rad51C-ATXN7 fusion protein in colon tumor cells. The immunoprecipitation pull down and immunoblot detection of the fusion protein Rad51C-ATXN7 in human colon cancer LS174T and RKO cell lines are shown. The pull down was carried out using 500 μg total cell lysates prepared from LS-174 T, RKO cells. Briefly, the total cell lysates were incubated with Dynabeads® containing protein G and 10 μg mouse monoclonal anti-Rad51C antibody. Immunoblot detection was carried out using Rabbit polyclonal anti-ATXN7 antibody and anti-rabbit secondary antibody by loading 150 μg total protein on 4–12 % NuPAGE gel. Lane 1- Total LS174T cell lysate, Lane 2- LS174T protein immunoprecipitated using anti-Rad51C antibody, Lane 3- Total RKO cell lysate, Lane 4- RKO protein immunoprecipitated using anti-Rad51C antibody. The immunoblot was detected using anti-ATXN7 antibody