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Fig. 2 | Molecular Cancer

Fig. 2

From: Roles of acid-extruding ion transporters in regulation of breast cancer cell growth in a 3-dimensional microenvironment

Fig. 2

Localization profiles of NHE1, NBCn1, MCT1 and MCT4 in MCF-7 and MDA-MB-231 spheroids. Sections of MCF-7 (A-D) and MDA-MB-231 (E-G) spheroids that were grown for 9 days followed by PFA fixation, embedding, and immunohistochemical analysis (IHC). a and e: NHE1 staining. b and f: NBCn1 staining. c: MCT1 staining. d and g: MCT4 staining. Images are representative of 3–5 n. Scalebars: 20 μm. L: indicates lumen of spheroid. h: Relative distribution of the transporters NHE1, NBCn1, MCT1 and MCT4 from the periphery towards the core (across the viable region) of MCF-7 spheroids. Based on two mean pixel intensity profiles per spheroid on three spheroids (only two for MCT1) from independent biological replicates per transporter/antibody (For details, see Methods)

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