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Fig. 6 | Molecular Cancer

Fig. 6

From: Combination of the novel histone deacetylase inhibitor YCW1 and radiation induces autophagic cell death through the downregulation of BNIP3 in triple-negative breast cancer cells in vitro and in an orthotopic mouse model

Fig. 6

BNIP3 knockdown by shRNA abrogates the growth of 4 T1 cells. a The expression levels of BNIP3 in 4 T1 cells were analyzed by western blot analysis following YCW and/or IR treatment. Cells were treated with YCW1 (1 μM) and IR (4 Gy) for 48 h. b Transfection efficacy was verified by western blot analysis. BNIP3 protein expression in 4 T1 cells transfected with scramble or BNIP3 shRNA for 24 h. c Quantitative data calculating the number of LC3 dots per cell transfected with scramble or BNIP3 shRNA. *, p < 0.05. d Cytotoxicity observed in cells transfected with scramble or BNIP3 shRNA using flow cytometry. *, p < 0.05. e Western blot analysis of BNIP3 protein expression in cells overexpressing BNIP3. f Quantitative data calculating the number of LC3 dots in the overexpression of BNIP3. Cells were transfected with BNIP3 for 24 h and were then incubated with YCW1 (1 μM) and IR (4 Gy) for 48 h. *, p < 0.05, YCW1 + IR versus BNIP3 + YCW1 + IR. Data are presented as the mean ± standard deviation of three independent experiments

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Molecular Cancer

ISSN: 1476-4598