Fig. 2
From: ErbB2 and p38γ MAPK mediate alcohol-induced increase in breast cancer stem cells and metastasis
Effect of alcohol on cancer metastasis in FVB MMTV Neu mice. a FVB MMTV Neu mice were fed with liquid diet containing ethanol (0 or 6.7 %) for about 12 months, blood alcohol concentration (BAC) was measured as described in Materials and Methods. b After the maximal diameter of tumors reached 20 mm, mice were sacrificed and analyzed for tumor metastasis as described in the Material and Methods. c and e The mammary tumor tissues were fixed, sectioned, and processed for immunofluorescent staining with indicated antibodies as described in the Material and Methods. Bar = 40 μm (c), 25 μm (e). d and f The intensity of CD44 (d), p-p38γ, CD44 or pErbB2 (f) in (c) and (e) were measured using ImageJ. The relative levels were quantified and normalized to the controls. g and h The mammary tumor tissues were collected and the expression of phosphorylated ErbB2 (pErbB2) and p38γ MAPK (p-p38γ) was determined by immunoblotting (N = 4). The relative levels of pErbB2 and p-p38γ were quantified and normalized to the loading control (Ponceau S stain). Each data point was the mean ± SEM of three independent experiments. *denotes significant difference from control groups (p < 0.05)