Fig. 3

Effect of alcohol on the activation of ErbB2 and p38γ MAPK. a MCF7-ErbB2 cells were exposed to alcohol (0, 100 or 200 mg/dl) for 10 days, then cell lysates were collected and the expression of phosphorylated ErbB2 and p38γ MAPK (pErbB2 and p-p38γ) was analyzed by immunoblotting. GAPDH served as loading controls. b Equal amount of proteins were immunoprecipitated (IP) with an anti-p38α/β antibody, then immunoblotted (IB) using an anti-pan-phosphorylated p38 MAPK antibody (p-p38). c MCF7-ErbB2 cells stably expressing control shRNA or shRNA for p38γ MAPK were exposed to alcohol (0 or 100 mg/dl) for 10 days, then cells lysates were collected. Equal amount of proteins were immunoprecipitated (IP) with an anti-SAP97 antibody, then immoblotted (IB) with antibodies directed against phosphorylated serine/threonine antibody and p38γ MAPK. The experiment was replicated three times