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Fig. 4 | Molecular Cancer

Fig. 4

From: MiR-143-3p functions as a tumor suppressor by regulating cell proliferation, invasion and epithelial–mesenchymal transition by targeting QKI-5 in esophageal squamous cell carcinoma

Fig. 4

MiR-143-3p negatively regulates the expression of QKI by directly targeting the QKI 3’-UTR. a Luciferase activity in cells following co-transfection with control or miR-143-3p-encoding plasmids and wild-type or mutant-type pMIR vectors harboring the predictive miR-143-3p binding site in the 3’-UTR of QKI. b Western blot analysis showed that miR-143-3p could inhibit the protein expression of ESCC cells. c Real time RT-PCR analysis of QKI expression in human ESCC tissue samples and their matched normal tissues from ESCC patients (n = 80). Expression levels are shown using a log2 scale. d RT-PCR analysis of QKI mRNA expression in ESCC cells compared with that in the normal esophageal epithelial cell line (HEEC). e Immunohistochemical staining of QKI protein in primary ESCC tissue samples (Left: ×100; Right: ×200). f Analysis of correlation between QKI and miR-143-3p expression levels in 80 pairs of ESCC tissue specimens. g RT-PCR analysis of the expression of the major QKI splice variants regulated by miR-143-3p. Values are mean ± SD; *: P <0.05; **: P <0.01

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