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Fig. 2 | Molecular Cancer

Fig. 2

From: Efficient elimination of pancreatic cancer stem cells by hedgehog/GLI inhibitor GANT61 in combination with mTOR inhibition

Fig. 2

Inhibition of hedgehog (Hh) signaling reduced the self-renewal as evidenced by sphere formation and the expression of stem cell marker CD133 of pancreatic cancer cells. a Representative photographs of Capan-1 M9 spheres cultured in a stem cell culture medium in the absence or presence of 20 μM of inhibitors, cyclopamine or GANT61. Scale bar: 100 μm. b The effects of cyclopamine and GANT61 on the number of spheres. Primary spheres were cultured in triplicate and the sphere numbers that were larger than 100 μm in diameter were counted per well. The results are presented as the mean and s.d. of triplicates obtained in 1 representative experiment out of 2.*P < 0.05. c The effects of GANT61 on the frequency of CD133+ cells. The results are presented as percentages of control values in untreated cells, showing the mean and s.d. of triplicates obtained in 1 representative experiment out of 2

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