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Fig. 4 | Molecular Cancer

Fig. 4

From: Efficient elimination of pancreatic cancer stem cells by hedgehog/GLI inhibitor GANT61 in combination with mTOR inhibition

Fig. 4

GLI1 knockdown reduces the cell viability and sphere formation of pancreatic cancer cells but not the colony formation in two dimensinal culture. a GLI1 expression after GLI1-si RNA transfection measured by RT-PCR. b Reduction of cell viability by GLI1-siRNA. Cells were transfected for 96 h, then followed by MTT assay. The results are presented as percentages of control values in untreated cells, showing the mean and s.d. of 4 replicates obtained in 1 representative experiment out of 2. c Inhibition of sphere formation by GLI1-siRNA. Cells were transfected for 96 h, then followed by single cell sphere formation assay. The results are presented as in Fig. 3c. d Effect of GLI1-siRNA on the colony formation in two-dimensional culture. Cells were transfected for 96 h, then single-cell suspensions were plated in 96 well plate as 1 cell/well. After 7 days incubation, the number of colonies formed in a plate were counted. The results are presented as the mean and s.d. of 3 replicates obtained in 1 representative experiment out of 2. *P < 0.05

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