Fig. 2
From: MiR-204 silencing in intraepithelial to invasive cutaneous squamous cell carcinoma progression
DNA methylation-associated silencing of miR-204 in cSCC cell lines and human samples. a Up, schematic representation of TRPM3/miR-204 gemomic locus. Down, DNA methylation levels of the miR-204 promoter region in cell lines and human biopsies. Methylation levels represent the mean of three consecutive CpGs (-26, -28, -40) located at the promoter region of miR-204. Error bars indicate standard deviation. b DNA methylation and expression levels of miR-204 in human biopsies detected by qRT-PCR. c Relative miR-204 levels in human epithelial keratinocytes and HaCaT cells, and restored expression of DNA methylated miR-204 in HaCaT cells after treatment with the DNA demethylating agent 5-aza-2′-deoxycytidine (Aza). Values were determined in triplicates by quantitative RT–PCR and are expressed as mean ± SD (n = 3)