Fig. 5

Effects of miR-204 overexpression on MAPK and STAT3 signaling pathways. a HaCaT cells were transiently transfected for 24 h with miR-204 or control mimics, and treated with EGF (10 ng/ml) for the indicated time points. Total extracts from these cells were subjected to western blot analysis (left). Intensity of individual bands was normalized to Actin signal, as a measure of protein relative abundance in the different samples and referred to control conditions, using Image J densitometry software (right panels). b Control or miR-204 mimic transfected HaCaT cells were treated with EGF, FGF9 or IL6 (10 ng/ml) for 24 h. Total extracts from these cells were subjected to western blot analysis (left). Intensity of individual bands was normalized to Actin signal, as a measure of protein relative abundance in the different samples and referred to control conditions, using Image J densitometry software (right panels). c HaCaT cells were treated with EGF, FGF9 or IL6 (10 ng/ml) for 24 h, in the absence or in the presence of 50 μM NSC87877, and processed as in b