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Fig. 2 | Molecular Cancer

Fig. 2

From: Proteinase-activated receptor 2 (PAR2) in hepatic stellate cells – evidence for a role in hepatocellular carcinoma growth in vivo

Fig. 2

PAR2 knockdown inhibits the pro-mitotic effect of LX-2 cell culture supernatants on Hep3B cells. LX-2 cells were treated on two consecutive days with transfection agent (Lipofectamine 2000) alone (-) or Lipofectamine 2000 plus 50 nM each of a universal control siRNA (Co) or siRNA to PAR1 or PAR2. After the second transfection, cells received normal growth medium which remained on the cells for 48 h. The resulting culture supernatants from PAR2 or PAR1 depleted and control LX-2 cells were then harvested, cleared by centrifugation and transferred to cultures of Hep3B cells. After a 72 h incubation period with the LX-2 conditioned media, Hep3B cells were detached and cell numbers determined by manual counting and trypan blue exclusion test. Data represent relative cell numbers (mean ± S.D.) from three independent experiments with cell numbers of Co cells set at 100 %. * p < 0.05 versus control

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