Fig. 4

LX-2 cells stimulated with PAR₂-AP secrete enhanced level of proliferation- and angiogenesis-associated cytokines and proteinases. LX-2-wt cells, serum-starved for 17 h were treated with PAR₂-AP (10 μM), trypsin (10 nM) or vehicle for 24 h and the supernatants were evaluated by protein analyses using a Human Angiogenesis Array Kit and b Human Protease Array kit as described in the Method section. In the representative images each pair of horizontal blots (bands) represents a different protein present in the supernatant, whereas the intensities of the blots characterised the amount of the respective protein (R = reference spot for protein loading). The results in the histograms below the images represent the mean ± SD for three independent experiments [**indicates a significant difference from non-stimulated (p < 0.05)]