Fig. 6

Pyk2 inhibition markedly attenuates ascites and CCL18-induced cell migration. a siRNA-mediated downregulation of Pyk2 attenuates ascites-induced migration of CaOV3 and OVCAR3 cells. Cells were transfected with non-targeted (NT siRNA) or Pyk2 siRNA. After 48 h, cells were transferred in Boyden chambers. OC ascites (OVC439 or OVC551) were used as chemoattractants in the lower chambers. Cell migration was measured 24 h later. b Immunoblot of CaOV3 cells transfected with NT or Pyk2 siRNA and probed with Pyk2 antibody. c CaOV3 and OVCAR3 cells were transfected with non-targeted (NT siRNA) or Pyk2 siRNA. Control corresponds to mock-transfected cells. After 48 h, cells were transferred in Boyden chambers. CCL18 (50 ng/ml) was used as chemoattractants in the low chambers. Cell migration was measured 24 h later. Results shown are representative of three independent experiments and data are expressed as fold increased relative to serum-free incubated cells. P values are indicated relative to mock and NT siRNA-transfected cells