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Fig. 2 | Molecular Cancer

Fig. 2

From: Rac1-mediated cytoskeleton rearrangements induced by intersectin-1s deficiency promotes lung cancer cell proliferation, migration and metastasis

Fig. 2

Subcellular distribution of ITSN-1s in LC cells. a Immunofluorescent staining with ITSN-1 Ab followed by AF488 Ab in A549 cells (a, a1) compared to bronchial cells (b, b1). b ITSN-1s protein expression was restored by stable transfection in A549 cells: myc-ITSN-1s protein level is detected via myc Ab in transfected A549 cells (a); lysates of bronchial cells, A549 cells and myc-ITSN-1s transfected A549 cells (A549 + ITSN-1s) were assessed for ITSN-1s protein level by WB using ITSN-1s Ab; actin used as loading control (b). c ITSN-1s protein level was quantified by densitometry. d Immunofluorescent staining of A549 + ITSN-1s cells with ITSN-1 Ab shows a similar subcellular distribution but increased intensity in A549 + ITSN-1s cells compared to A549 cells (d, d1). Error bar represents mean ± SE; *p < 0.05. Data are representative of 3 independent experiments performed under identical experimental conditions. Images were acquired using identical parameters. Bars: 20 μm (a, b, d); 5 μm (a1, b1, d1)

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