Fig. 3
ITSN-1s impairs LC cell proliferation and growth. a A549 and A549 + ITSN cells (107) were seeded in 100 cm2 plates, cultured for five days, trypsinized and counted. b Serial dilutions of A549 and A549 + ITSN-1s cells seeded in 96-well plates were subjected to the MTT assay. Cell proliferation was assessed by absorbance at 570 nm; (w-well). c Three points on the linear part of the proliferation curve were used to evaluate the extent of cell growth inhibition. Data are shown as percentage of control. d A549 and A549 + ITSN-1s cells (5×105) were seeded in 35 mm dishes in media with 0.28 % agar on a base of 0.58 % agar. Colonies began to form 30 days later, and were stained with crystal violet dye. Representative plates are shown. e The number of colonies was quantified. Data are shown as percentage of control. Error bar represents mean ± SE; *p < 0.05; **p < 0.01. The above data are representative of 3 independent experiments performed under identical experimental conditions. f A549 and A549 + ITSN cells (107) injected into nude mice. Shown are representative tumors that formed 30 days post-injection. g Mean tumor area was measured using NIH ImageJ. Error bar represents mean ± SE; (n = 3-5 mice); *p < 0.05