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Fig. 4 | Molecular Cancer

Fig. 4

From: Rac1-mediated cytoskeleton rearrangements induced by intersectin-1s deficiency promotes lung cancer cell proliferation, migration and metastasis

Fig. 4

ITSN-1s impairs LC cell migration and metastasis. a A549 and A549 + ITSN-1s cell layer was wounded using a sterile micropipette tip and cells at multiple points along the wound edge were imaged every 15 min over a 48 h period using time-lapse microscopy. Bar: 10 μm. b Measurements were made using NIH ImageJ. Scratch closure was expressed as a percentage of initial scratch area in each well. c Scratch closure in mitomycin C treated and untreated A549 and A549 + ITSN-1s cells were compared. Data are shown as percentage of control. Error bar represents the mean ± SE; **p < 0.01. Data are representative of 3 independent experiments performed under identical experimental conditions. d Histological analyses of lung metastasis in immunodeficient mice injected retroorbitally with A549 and A549 + ITSN-1s cells. Bar: 100 μm. e, f Total number as well as the size of the lung metastasis per section was determined. Data are shown as median ± SE; **p < 0.01 (n = 5-7 mice). 5 lung sections per mice were examined using identical parameters

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Molecular Cancer

ISSN: 1476-4598