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Fig. 5 | Molecular Cancer

Fig. 5

From: Rac1-mediated cytoskeleton rearrangements induced by intersectin-1s deficiency promotes lung cancer cell proliferation, migration and metastasis

Fig. 5

ITSN-1s increases actin bundles and number of FAs. Representative phalloidin staining of A549 (a) and A549 + ITSN-1s cells (b). Thick actin filaments were often detected in A549 + ITSN-1s wells (B, boxed area, b1). Cell lysates were subjected to differential centrifugation to obtain the G-actin and F-actin fractions, which were analyzed by WB with actin Ab followed by densitometry (c). Representative double fluorescent staining with phalloidin (d, g) and vinculin/AF594 Ab (e, h) of A549 + ITSN-1s and A549 cells respectively. Co-localization of actin/vinculin is significant in A549 + ITSN-1s (f, f1). Limited co-localization is detected in A549 (i). The number of vinculin clusters/250 μm2 cell surface was counted (j). Data are representative of 3 independent experiments performed under identical experimental conditions. 50 cells per experiment were analyzed. Images were acquired using identical parameters. Bars: 10 μm (A-I); 5 μm (b1, f1). Data are shown as mean ± SE; **p < 0.01

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Molecular Cancer

ISSN: 1476-4598