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Fig. 3 | Molecular Cancer

Fig. 3

From: Esculetin induces antiproliferative and apoptotic response in pancreatic cancer cells by directly binding to KEAP1

Fig. 3

Esculetin induces loss of Mitochondrial membrane potential: a Flow cytometric analysis of PANC-1 cells stained with JC-1 dye after 100 μM esculetin treatment for indicated time showed a temporal decrease in ratio of red fluorescence (JC-1 aggregates) to green florescence (JC-1 monomers). b Percentage of cells exhibiting monomers and aggregates of JC-1 after treatment of cells with esculetin for different time intervals. c Western blot analysis showing temporal increase in cytosolic cytochrome C in PANC-1 cells. (VC stands for vehicle control, E stands for esculetin treatment sample for indicated time, CCCP stands for positive control i.e., carbonyl cyanide 3-chlorophenylhydrazone treated cells, numerals represent time of esculetin treatment). Data represents the mean ± SD of three independent experiments. The significance was determined using ANOVA (Bonferroni’s test). Key:*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001)

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Molecular Cancer

ISSN: 1476-4598