Fig. 4

Treatment with plerixafor results in decreased intratibial tumor growth when administered concurrently with tumor cell injection. a Diagram of experiment timeline. On Day 0, PC-3 M-luc2 cells were injected intratibially and saline control or plerixafor treatment was started via an osmotic pump. b After 21 or 23 days, in vivo luciferase imaging was performed. Images shown are taken at either 21 or 23 days. c Tumor growth at 21 or 23 days post injection of cells was determined by in vivo luciferase imaging (n = 6). * Represents p < 0.05. d Ex vivo x-ray imaging of media injected (left) and tumor bearing (right) tibiae was performed at 22 and 24 days post-injection. e H&E was performed on tissue sections from PC-3 M-luc2 intratibial tumors treated with saline control or plerixafor. Representative images were taken at 5X and digitally merged. f H&E staining was used for bone histomorphometric analysis to determine the ratio of cortical bone (left), trabecular bone (middle), and tumor cells (right) in each tissue section. g Tissue sections from PC-3 M-luc2 intratibial tumors were immunostained with anti-CXCR4 antibody. Images were taken at 20X and 40X (insert). h Proliferation of PC-3 M-luc2 tumors was analyzed by Ki67 staining; an average number of Ki67+ cells from five 20X fields was determined