Fig. 2

miR-375 induced docetaxel resistance in PC cells. a After miR-375 lentivirus transfection, both DU-145 and PC-3 cell lines show stably high level expression. b PC cells were treated with different concentrations of docetaxel for 48 h. Cell proliferation assay shows that cells with miR-375 transfection are more tolerant to docetaxel toxic effect. c 24 h after miR-375 transfection, cells were treated with docetaxel (50 nM) for another 24 h. Flow cytometry analyses show that high levels of miR-375 protect cells from apoptosis in both PC-3 and DU145 cell lines. Fraction of apoptotic cells are shown in lower right corners