Fig. 1
PI3 kinase pathway regulated miRNAs in glioblastoma a, b. Western blot data indicating the time course loss of phosphorylation of AKT upon PI3 kinase inhibition by LY294002 (50 μM) treatment in two different cell lines U87, and U251 respectively. Also indicated is the densitometric quantification of p-AKT/AKT levels at different time points. The first bar of each plot represents the untreated (UT) condition, where equal volume of DMSO was added as vehicle control. The quantitation and the statistics in the column plots has been deduced from three independent experiments. p values were calculated by student’s t-test and the symbols are indicated. (**) p ≤ 0.01; (***) p ≤ 0.001. c. Volcano plot representing the significantly regulated miRNAs upon LY294002 treatment, 15 miRNAs (indicated by red dots) were found to be upregulated upon PI3 kinase pathway inhibition and 1 (indicated by green dots) miRNA was upregulated. miRNAs were considered significant based on their p value and differential Log2 ratio (more than +/− 0.57 fold). d. Heat map of the 47 miRNAs derived from the volcano plot which were significantly different upon LY294002 treatment in U87 and U251 cell lines