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Fig. 4 | Molecular Cancer

Fig. 4

From: PI3 kinase pathway regulated miRNome in glioblastoma: identification of miR-326 as a tumour suppressor miRNA

Fig. 4

Arrestin beta 1, the host gene of miR-326 is also regulated by PI3 kinase pathway and is downregulated in GBM. a. Schematic representation of the genomic locus of MIR326 gene in the intronic region of ARRB1 gene. MIR326 resides in the first intron of ARRB1. b, c. Log2 transformed ARRB1 expression values obtained from different publicly available expression data sets, TCGA i (Affymetrix), TCGA ii (Agilent), GSE22866 and REMBRANDT data set respectively. Each dot represents one sample and for each sample fold change in expression is calculated over its mean expression in control brain samples. p values were calculated by student’s t-test or ANOVA (c) and the symbols are indicated. (*) p ≤ 0.05; (***) p ≤ 0.001. d, e. Correlation graph between hsa-miR-326 and ARRB1 transcripts using two different expression platforms from TCGA agilent (d) and affymetrix (e). ARRB1 and miR-326 exhibit a significant positive correlation in their abundance with a correlation coefficient R = 0.2446 and p < 0.0001 (agilent) and R = 0.2137 and p < 0.0001 (Affymetrix). f. Log2 transformed expression values of ARRB1 upon PI3 kinase pathway inhibition with LY294002 treatment (50 μM) in two different cell lines LN229 and U87 for two time points (6, 12 h)

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