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Fig. 6 | Molecular Cancer

Fig. 6

From: Tryptophan hydroxylase 1 and 5-HT7 receptor preferentially expressed in triple-negative breast cancer promote cancer progression through autocrine serotonin signaling

Fig. 6

Inhibitory effect of BJ-1113 on Gαs and Gβγ signaling in MDA-MB-231 breast cancer cells. a, b Inhibitory effects of BJ-1113 on cAMP levels were investigated using a cAMP ELISA kit. In a, cAMP level was measured in cells treated with 5-HT in the absence or presence of BJ-1113. *P < 0.05 vs. vehicle-treated controls. # P < 0.05 vs. 5-HT-treated cells. In b, BJ-1113 inhibited cholera toxin- and pertusis toxin-induced cAMP in MDA-MB-231 cells. *P < 0.05 vs. vehicle-treated controls. # P < 0.05 vs. cholera toxin- or pertusis toxin-treated cells. c Inhibitory effect of BJ-1113 on the activation of Gβγ-downstream signaling in 5-HT-stimulated MDA-MB-231 cells. The results in bar graphs represent the relative densities of each signaling protein of phospho-to-parent and are the means ± SEMs of three independent experiments. *P < 0.05 vs. vehicle-treated controls. # P < 0.05 vs. 5-HT-treated cells

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