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Fig. 3 | Molecular Cancer

Fig. 3

From: STAT3 signaling drives EZH2 transcriptional activation and mediates poor prognosis in gastric cancer

Fig. 3

EZH2 mediated anti-apoptotic effects in STAT3 signaling pathway. siSTAT3 and EZH2 inhibitor, DZNep, suppressed cell proliferation through apoptosis and cell cycle arrest. a DZNep, as the specific EZH2 inhibitor, induced cell apoptosis with a dose-dependent manner (P = 0.0064). b siSTAT3 and/or DZNep (500 nM), decreased cell viability, as determined by MTT assay (P = 0.0052). c Effects of siSTAT3 transfection and/or DZNep exposure on cell viability for 48 h by MTT assay. Decreased cell viability was clearly detected in the group treated with DZNep and/or siSTAT3 compared with the relative negative control (DZNep vs. Vehicle (0.1% alcohol, solution for DZNep), P = 0.0183; siSTAT3 vs. siNC, P = 0.0026, DZNep + siSTAT3 vs. Vehicle, P < 0.001; DZNep + siSTAT3 vs. siNC, P < 0.001). d The apoptotic rate was increased upon DZNep treatment and/or siSTAT3 transfection by FITC-Annexin-V/PI staining (DZNep vs. Vehicle, P = 0.0023, siSTAT3 vs. Vehicle, P =0.0035; DZNep + siSTAT3 vs. Vehicle, P = 0.001, respectively). e Caspase-3 activity in cells treated with SGC7901, siNC, DZNep, siSTAT3, or a combination of DZNep and siSTAT3. The activity of caspase-3 was higher in the group treated with siSTAT3 and/or DZNep treatment than in cells treated with vehicle (DZNep vs. Vehicle, P = 0.0056; DZNep + siSTAT3 vs. Vehicle, P = 0.0029) and siNC (siSTAT3 vs. siNC, P = 0.0215; DZNep + siSTAT3 vs. siNC, P = 0.0038). f Caspase-9 activity in cells treated with SGC7901, siNC, DZNep, siSTAT3, or a combination of DZNep and siSTAT3. The activity of caspase-9 was higher in the group treated with siSTAT3 and/or DZNep treatment than in cells treated with vehicle (DZNep vs. Vehicle, P = 0.0033; DZNep + siSTAT3 vs. Vehicle, P = 0.0012) and siNC (siSTAT3 vs. siNC, P = 0.0137; DZNep + siSTAT3 vs. siNC, P = 0.0214). g Cell cycle remained unchanged treated by DZNep alone in cells. All results in (a), (b) and (c) are presented as the mean ± SD values of three assays

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