Fig. 1
From: Acidic tumor microenvironment abrogates the efficacy of mTORC1 inhibitors
Acidic extracellular pH reduces the antiproliferative efficacy of rapamycin. a HT29 cells were cultured in medium buffered to pH 7.4, 6.8 or 6.4 and treated with rapamycin (100 nM), MK-2206 (1 μM), U0126 (10 μM) or DMSO for control. MTS proliferation assay was performed after 48 h of treatment. Bar charts represent mean, error bars represent SD. * p < 0.05, Student’s t test compared to control cells at the same pH. b 786–0, Caki-1, Huh7, PLC-PRF/5 and MC-38 cells were cultured at pH 7.4 or 6.4 and treated with rapamycin (100 nM) or DMSO for control. MTS proliferation assay was performed after 48 h of treatment. Bar charts represent mean, error bars represent SD. * p < 0.05, Student’s t test compared to control cells at the same pH