Fig. 6
From: Acidic tumor microenvironment abrogates the efficacy of mTORC1 inhibitors
Effects of rapamycin and bicarbonate on tumor necrosis, mTORC1 activity, tumor apoptosis and proliferation. Percentage of tumor necrosis (light pink stained surface in H & E shown in Fig. 5) and phospho S6 expression (phospho S6 positive surface shown in Fig. 5) were compared for vehicle, sodium bicarbonate, rapamycin, and combined treatment in HT29 xenografts and MC-38 allografts in 10 representative sections of 3368 × 2668 μm for three different tumors using ImageJ Threshold Colour Plugin analysis. Percentage of PCNA positive cancer cells and percentage of cleaved caspase 3 positive cancer cells was counted in 10 representative zones of 100 × 100 μm for three different HT29 and MC-38 tumors. Bar charts represent mean, error bars represent SD. **** p < 0.0001, *** p < 0.001, ** p < 0.01, * p < 0.05, ns = not significant, One-way ANOVA