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Fig. 5 | Molecular Cancer

Fig. 5

From: Lipocalin2 suppresses metastasis of colorectal cancer by attenuating NF-κB-dependent activation of snail and epithelial mesenchymal transition

Fig. 5

LCN2 suppresses the NF-κB/snail signaling pathway and attenuates NF-κB promoter activity. a Western blots detection of p65 expression in LCN2-overexpressing and LCN2-knockdown cells. b Western blots detection, quantification of relative mRNA expression of snail expression in corresponding cells. c Immunofluorescence of snail expression and location in the indicated cells. d, e Western blots detection of EMT markers and snail changes (d) and quantification of relative mRNA expression of EMT markers (e) after treatment with LMB (20nM for 10 h) and Bay11-7082 (50 μM for 3 h) in corresponding cells. The grey value ratio of Vimentin/GAPDH was shown. f Survival analysis (Kaplan-Meier method, log-rank test) of combined LCN2 and NF-κB expression in CRC patients (n = 126; group 1 (n = 21, 3 died) vs group 2 (n = 48, 17 died), P = .083; group 1 vs group 3 (n = 27, 10 died), P = .088; group 1 vs group 4 (n = 30, 13 died), P = .042). Values shown in real-time PCR assay are the mean ± SD from at least three independent experiments. (N, nuclear; P-phosphorylated). * P < .05, ** P < .01

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