Fig. 1

SAG is crucial for progression of prostate cancer in human and mice. (a & b) Expression of SAG is assessed in a human prostate cancer TMA. SAG staining indexes in cohorts of normal prostate epithelial cells (n = 24) and prostate tumors (n = 58) are shown as stacked columns. (c & d) PB4-Cre inactivates Pten and Sag in prostate epithelium cells. Prostate tissues from mice at age of 6 months with indicated genotypes were fixed in 10% formalin, embedded, sectioned and stained with Pten and Sag Abs. (e) Western blotting for Pten and Sag in prostate tissues (four independent samples with two genotypes). (f) Haematoxylin and eosin (h & e) staining of the prostate tissues from mice at age of 6 months with indicated genotypes. Scale bar represents 100 μm. (g) Pie graphs show prostate tumor progressions in Pten ^PC-/-;Sag ^+/+ and Pten ^PC-/-;Sag ^PC-/- mice at age of 6 months (n = 10). HGPIN, high-grade PIN; LGPIN, low-grade PIN. The quantitative results of tumor progression are from two randomly selected slides of each mouse in a total 10 pairs of the entire animal cohort. (h) Mice with indicated genotypes were sacrificed at ages of 3, 6, and 9 months, respectively. The weight of prostate vs. whole body were weighed, the % weight was calculated and plotted (n = 6). * p < 0.05. (i & j) Prostate tissues with indicated genotypes were stained for Ki67 with representative images shown (i). Positive cells were counted from at least 3 randomly selected microscopic fields with % positivity calculated (j). * p < 0.05. Scale bar: 100 μm