Fig. 2

SAG Knockdown suppresses growth, survival and migration of human prostate cancer cells via inactivation of the PI3K/AKT/mTOR axis. DU145 human prostate cancer cells were infected with Lenti-SAG (Lt-SAG), along with Lenti-GFP (Lt-Con) as a control for 72 hrs. Cell proliferation was measured by ATP-lite assay (a), clonogenic survival (b), soft agar assay (c), and Boyden chamber migration assay (d), as well as western blotting assay using indicated Abs (e). Shown are mean ± SEM from three independent experiments (a-d). * p ; ** p < 0.01. Scale bar represents 50 μm