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Fig. 6 | Molecular Cancer

Fig. 6

From: Depletion of SAG/RBX2 E3 ubiquitin ligase suppresses prostate tumorigenesis via inactivation of the PI3K/AKT/mTOR axis

Fig. 6

Sag-SCFβTrCP modulates the PI3K/AKT/mTOR axis during mouse prostate tumorigenesis driven by Pten loss. a-c Accumulation of Phlpp1 and Deptor, and reduction of pAkt, pS6 and p4Ebp1: (a & b) Prostate tissues from mice at age of 6 month with indicated genotypes were stained with indicated Abs. Shown are representative areas of stained tissues (left panel), and the staining was analyzed using 3D Histech software and plotted (right panel). All values represent means ± SD; n ≥ 5 for each group. *p ≤ 0.05; **p ≤ 0.01. Scale bars: 100 μm. c Cell lysates from prostate tissues with indicated genotypes (4 independent samples from two genotypes, respectively) were analyzed by Western blotting with indicated Abs. d Working Model: Sag deletion blocks prostate tumorigenesis triggered by Pten loss. The PI-3 K/AKT/mTOR axis was activated upon Pten loss to trigger prostate tumorigenesis, whereas Sag deletion caused accumulation of Phlpp1 to inactivate Akt, and of Deptor to inactivate mTOR, leading the suppression of prostate tumorigenesis

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