Fig. 3

The role of miR-138-5p targeting Survivin in regulating the proliferative and invasive abilities of bladder cancer cells. a The CCK-8 viability assay was performed 12, 24, 36, and 48 hours after T24 cells were transfected with miR-138-5p mimic (mim-miR-138-5p), miR-138-5p inhibitor (anti-miR-138-5p) or corresponding scrambled negative control RNA (mim-scramble and anti-scramble, respectively). The viability of the experimentally treated cells were compared to that of their associated control-treated cells (i.e., mim-miR-138-5p vs mim-scramble; anti-miR-138-5p vs anti-scramble). b The CCK-8 viability assay was performed 12, 24, 36, and 48 hours after T24 cells were transfected with mim-scramble plus control vector (pCDNA3.1), mim-miR-138-5p plus control vector, mim-scramble plus BIRC5 plasmid (pCDNA3.1-BIRC5), or mim-miR-138-5p plus BIRC5 plasmid (pCDNA3.1 + mim-scramble vs pCDNA3.1-BIRC5 + mim-scramble, and pCDNA3.1 + mim-miR-138-5p vs pCDNA3.1-BIRC5 + mim-miR-138-5p, respectively). c and e, transwell analysis of invading T24 cells treated with equal amounts of mim-miR-138-5p, anti-miR-138-5p, mim-scramble or anti-scramble. (c): representative image; (e): quantitative analysis. d and (f), transwell analysis of invading T24 cells treated with equal amounts of mim-scramble plus control vector (pCDNA3.1), mim-miR-138-5p plus control vector, mim-scramble plus BIRC5 plasmid (pCDNA3.1-BIRC5), or mim-miR-138-5p plus BIRC5 plasmid. (d), representative image; (f), quantitative analysis. *p < 0.05; ** p < 0.01; *** p < 0.005