Fig. 5

Alteration of SLC34A2 levels influences the in vitro effects of miR-939 in GC cells. a protein levels of overexpressed SLC34A2 lacking 3'UTR in miR-939-overexpressing GC cells, as assessed by western blot. b and c MTT analysis (b) and colony formation assay (c) indicated that restoration of SLC34A2 in miR-939-overexpressing GC cells recovered cellular proliferation and tumorigenicity ability. d Annexin V/PI staining assays showed that restoration of SLC34A2 in miR-939-overexpressing GC cells decreased 5-Fu-induced apoptosis. e the migratory capacity of miR-939-overexpressing GC cells was enhanced after transfected with full-length SLC34A2, as assessed by a wound-healing assay. f overexpression of SLC34A2 in miR-939-transfected cells increased the number of invaded cell, as assessed by a Matrigel invasion assay. g in GC cells with miR-939 overexpression, the expression levels of p-ERK (lane 2) were significantly decreased compared with the control (lane 1). Restoration of SLC34A2 abrogated the decreased expression of p-ERK induced by miR-939 in GC cells (lane 3). Western blot analysis demonstrated that ERK inhibitor LY294002 (LY) could effectively decrease expressions of p-ERK induced by SLC34A2 (lane 4). h western blot analyses show that the expression of C-Raf and p-MEK was decreased in miR-939-transfected cells, while increased after SLC34A2 overexpressed