Fig. 1

BAP1 protein and mRNA expression in CCA cells. a: BAP1 mRNA expression by quantitative RT-PCR (qRT-PCR). Data represents mean ± SEM of 2^-dCt values normalized to respective β-actin RNA (n = 3). b: Representative western blots and semi-quantitative analysis following normalization to PCNA of BAP1 protein expression in nuclear (N) or cytosolic (C) fractions under baseline conditions, from three independent studies. c: Western blot analysis of BAP1 protein expression in parental HAP1 cells (WT) and in HAP1 cells with CRISPR-Cas9 mediated knock-out of BAP-1 (BAP1 KO) The graph shows the mean ± SEM of 2^-dCt values from qRT-PCR expression analysis of BAP1 mRNA normalized to respective β-actin RNA (n = 3). * P < 0.05. d: BAP1 protein expression in H69 cells by immunocytochemistry and Western blot analysis in nuclear fraction of non-malignant H69 cells. Histone H3 was used as a reference control. BAP1 antibody was purchased from Santa Cruz (SC-28383) and used at a dilution of 1:250