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Fig. 4 | Molecular Cancer

Fig. 4

From: BAP1 dependent expression of long non-coding RNA NEAT-1 contributes to sensitivity to gemcitabine in cholangiocarcinoma

Fig. 4

Synergistic interactions between candidate agents with gemcitabine. a: Cytotoxicity to drugs was assessed in KMBC (blue circles) and HuCCT1 (purple squares) cells incubated with gemcitabine (Gem) in combination with trichostatin A (TSA), b-AP15, olaparib, or GSK126 in a fixed ratio of concentrations, based on the IC50 of each drug. Potential interactions were evaluated using the median effects analysis of Chou and Talalay, and the combination index (CI) derived, with a CI < 1 indicative of a synergistic and a CI > 1 indicative of an antagonistic effect. At a fractional effect of 0.75, a synergistic effect was observed between Gem and TSA or b-AP15 in HuCCT1 cells but not with olaparib or GSK126, whereas the converse was noted in KMBC cells. b: KMBC cells grown in three-dimensional multicellular spheroids and incubated with gemcitabine. Representative morphological images in spheroid culture (top) and comparison of concentration dependent effects on cell viability cells in conventional in vitro or spheroid culture (bottom). The IC50 was 25nM in conventional and 1258nM in the spheroid cultures. c: Combination index from median effects analysis of combination treatment with gemcitabine and GSK126 in KMBC cells cultured under either conventional (conv) or multicellular spheroid culture. A synergistic effect was observed with cells in spheroid culture, similar to conventional (conv) in vitro culture conditions

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