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Fig. 6 | Molecular Cancer

Fig. 6

From: Quercetin-induced miR-200b-3p regulates the mode of self-renewing divisions in pancreatic cancer

Fig. 6

MiR-200b-3p triggers cell differentiation. a Dissociated AsPC1, AsanPaca and PANC1 cells were cultured in regular medium (DMEM, 10% FCS, 1% HEPES). The cells were treated with 50 nM of miR-200b-3p or miR control (miR-NC) mimics for 72 h or were treated with quercetin 50 μM for 12 h (Q) or were left untreated (CO) for every 72 h for a period of 12 days and subsequently evaluated for osteogenic differentiation. Heterogeneous osteogenic differentiation was detected using Sigma Fast BCIP/NBT substrate. The positive cells appear deep bluish purple. b Dissociated AsPC1, AsanPaca and PANC1 cells were treated as described above and evaluated for adipogenic differentiation. Heterogeneous adipocytes were stained red by the Oil Red O staining solution and visualized with a 10 × magnification using an inverted microscope c Dissociated AsPC1, AsanPaca and PANC1 cells were treated as described above and evaluated for chrondrogenic differentiation. Heterogeneous chondrogenic differentiation was detected as intense dark-blue coloration while the other cells were mostly light bluish. The bar indicates 100 μm

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