Fig. 2

Silencing of CD73 inhibits NSCLC cell proliferation and motility. a CD73 mRNA and protein levels in stable A549 and H226 cells. b CCK-8 assay of cell viability in NSCLC cell lines; cell viability was determined at 24, 48 and 72 h. c Representative images of clonogenic analysis of cell proliferation in NSCLC cells. Bar charts showing clonogenic growth of cells. d and e Flow cytometric analysis of NSCLC cell lines (CD73-silenced cells vs. NC cells). Cells were harvested at 72 h after transfection and stained with propidium iodide. The percentage of cells in each cell cycle phase is shown in the inset of each panel, in which the values represent the mean ± SD of three measurements. f and g Wound healing assay was performed to observe the role of CD73-silenced cells, the speed with which cells migrated towards the scratch was lower in sh-CD73-transfected cells than in control cells. h and i CD73 silencing inhibits invasion and migration of NSCLC cells. CD73-silenced NSCLC cells were allowed to migrate through an 8-μM pore Transwell. The cells that migrated were stained and counted in at least three microscopic fields (magnification, ×100). Then, the cells were treated as above and allowed to invade through the Matrigel-coated membrane in Transwells. Invasive cells were stained and counted under a light microscope. ^* P < 0.05; ^** P < 0.01; ^*** P < 0.001