Skip to main content
Fig. 5 | Molecular Cancer

Fig. 5

From: CD73/NT5E is a target of miR-30a-5p and plays an important role in the pathogenesis of non-small cell lung cancer

Fig. 5

Overexpression of miR-30a-5p inhibits NSCLC cell proliferation and motility. a & b CCK-8 assay of cell viability in NSCLC cell lines transfected with miR-30a-5p mimics or miR-30a-5p inhibitor at 24, 48 and 72 h. c A wound healing assay was performed to observe the role of miR-30a-5p transfection in cells. The data showed that the speed with which the cells migrated towards the scratch was lower in cells transfected with the miR-30a-5p overexpression vector than in the control cells. d Overexpression of miR-30a-5p inhibits invasion and migration of NSCLC cells. The A549 and H226 cell lines were transfected with miR-30a-5p mimics and allowed to migrate through 8-μM pore Transwells. The cells that migrated were stained and counted in at least three microscopic fields (magnification, ×100). Then, cells were treated as described before and allowed to invade through the Matrigel-coated membrane in Transwells. The invasive cells were stained and counted under a light microscope. e and f The A549 and H226 cells were treated with or without miR-30a-5p mimics or the miR-30a-5p inhibitor for 72 h, respectively. The expression levels of p-EGFR, EGFR, p-AKT, AKT, p-ERK, ERK and Cyclin D1 were analyzed by western blotting. ** P < 0.01; *** P < 0.001

Back to article page